Medium refers to the growth and reproduction of microorganisms, plants, or animals (or tissues), and the combination of different nutrients into a nutritional matrix. Generally contain carbohydrates, nitrogen, inorganic salts (containing trace elements), vitamins, and water. Medium is not only the basic material to provide cell nutrition and promote cell proliferation, but also the living environment for cell growth and reproduction. There are many kinds of media: According to the source of raw materials, it can be divided into natural medium, synthetic medium, and semi-synthetic medium. According to the physical state can be divided into solid medium, liquid medium, and semi-solid medium; Basic medium, selective medium, enrichment medium, identification medium, etc. According to the scope of use can be divided into bacterial culture medium, actinomycetes culture medium, yeast culture medium, fungal culture medium. After preparing the medium, the pH value generally needs to be tested and adjusted, but also must be sterilized, usually by high-temperature sterilization and filtration sterilization, the medium is rich in nutrients, vulnerable to contamination or deterioration. Therefore, do not place the device for a long time after it is installed. It is best to use it immediately. The liquid filled Peristaltic Pump is mainly used for high precision liquid distribution of medium Media are usually sterilized in triangular flares or test tubes. For static culture, take 100ml medium / 250ml triangle flask to 150ml medium / 250ml Triangular bottles, otherwise in the disinfection process boiling medium easy to contaminate the tampon, resulting in bacterial breeding; The 15-20ml/250ml triangular flask was well ventilated during shaker culture. The liquid medium is usually filled with 4-5ml, about 1/4 of the height of the tube The commonly used media are: milk AGAR medium/rhizobia medium/bovine heart medium/actinomycetes medium/flour AGAR medium/microalgae medium/selenium medium/Pr medium/fungus medium/edible fungus medium/tobacco medium/animal cell medium/selective medium.
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