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Application of peristaltic pump in chromatography?

2021-09-30

Chromatography is a kind of mixture separation technology, which is widely used in analytical chemistry, organic chemistry, biochemistry, and other fields. Chromatography uses the difference of physical and chemical properties of each component in the mixture to separate and determine the multi-component mixture. According to the chromatography mechanism, it can be divided into adsorption chromatography, distribution chromatography, ion-exchange chromatography, gel filtration chromatography, and so on.

Under natural conditions, limited by liquid chromatography column in the penetration speed, needed a suitable external pressure, in order to strengthen the infiltration of liquid capacity, at the same time also need to ensure that the liquid without cross-contamination Peristaltic Pump, therefore, is the ideal choice of the transmission fluid in the process of chromatography, are often used to control the flow rate of the mobile phase velocity eluent pumping buffer extraction under test samples, etc.

To ensure product cleanliness after filtration/chromatography, it is necessary to avoid secondary contamination of the fluid by the equipment in the system during filtration/chromatography. Peristaltic pumps are ideal for pumping fluids in such processes:

1. Peristaltic pump can carry out high precision and stable fluid transmission, and can maintain stable fluid pressure in the transmission process.

2. When the peristaltic pump transmits the fluid, the fluid only contacts the hose. Select the hose that meets the hygiene requirements to avoid secondary pollution of the fluid in the transmission process. To transfer different fluids, simply replace the hose to avoid cross-contamination between different fluids.

3. Peristaltic pump is suitable for the transmission of liquid containing solid particles. When used together with the filter, it can effectively remove particles or other suspended objects in the fluid, which will not wear the pump body and is simple to maintain.

4. Peristaltic pump has low shear force, which can not be replaced by other pumps for the low loss transmission of biologically active substances in the fluid.


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